Pr Eric E. GabisonOphthalmology · Cornea & refractive · Paris
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HomeCoursesCorneal transplantation › Penetrating keratoplasty & DALK
Course contents ▾
  1. Evolution & paradigm
  2. Applied anatomy
  3. Epidemiology 2026
  4. Taxonomy
  5. Tissue banks
  6. Immunology & rejection
  7. Penetrating KP
  8. Anterior lamellar
  9. Endothelial
  10. Regenerative turn
  11. Keratoprostheses
  12. Surface & limbus
  13. Complications & follow-up
  14. Special situations
  15. Perspectives & synthesis
Chapter 07

Penetrating keratoplasty (PK / PKP)

The historic procedure, now reserved for situations where no selective approach is possible: opacity involving the full thickness, a highly irregular or thinned cornea, perforation, or multiple lamellar failures.

Principle & technique

Trephination of a recipient button (often 7.5-8.25 mm) and of a slightly larger donor graft, then suturing (interrupted, running, or a combination) under the microscope. Managing post-graft astigmatism — through suture tension, intraoperative keratometry and selective suture removal — is the central challenge of visual recovery.

Contribution of the femtosecond laser

The femtosecond laser allows profiled cuts (zig-zag, mushroom, top-hat edges) that increase the healing surface, improve apposition and junction strength, and can speed visual rehabilitation compared with a vertical trephine cut.

Penetrating keratoplasty — assessment
AdvantagesLimitations
Universal solution (any layer), tectonicFrequent irregular astigmatism
Maximum track record and standardisationHighest endothelial rejection; long-term sutures
Essential for perforation/total opacitySlow visual recovery; tectonic fragility (lifelong risk of traumatic dehiscence)

▶ Watch corneal graft videos (surgical technique & reports) on egabison.com

Chapter 08

Anterior lamellar keratoplasty (ALK / DALK)

When the endothelium is healthy but the stroma is diseased — the emblematic case of keratoconus — DALK replaces the stroma down to Descemet's membrane while preserving the native endothelium. The cardinal benefit: elimination of endothelial rejection and therefore potentially lifelong graft survival.

Anwar's big bubble

The reference technique: after partial trephination and stromal dissection, air/viscoelastic is injected to cleave the deep stroma from Descemet's membrane.

  • Type 1 — central bubble, cleavage at the Dua / pre-Descemet layer: robust bed, the most favourable.
  • Type 2 — large peripheral bubble beneath true Descemet: more fragile wall, increased perforation risk.
  • Mixed — coexistence of both, to be recognised to avoid rupture.

Alternatives: viscodissection, manual layer-by-layer dissection (Melles), femtosecond assistance for trephination and cutting planes.

Learning curve & conversion

DALK is technically demanding: micro-perforation of Descemet's membrane is the key intraoperative complication and may force conversion to penetrating keratoplasty. Visual results, when dissection reaches the pre-Descemet plane, equal those of PK, without the endothelial risk. Main indications: keratoconus, ectasias, stromal scars and dystrophies, post-infectious opacities not reaching the endothelium.

2026 marker — additive rather than replacement

For keratoconus, a growing share of cases now falls to CAIRS (allogenic corneal intrastromal ring segments): human corneal tissue is added within a stromal tunnel to remodel the surface, with no synthetic material and no penetrating graft. Planning relies on dedicated nomograms (e.g. Jacob, Istanbul). See also Ch. 10.

▶ Watch the “double docking” DALK technique videos (egabison.com)